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Invitrogen

Mouse Albumin ELISA Kit

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Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Mouse Albumin ELISA Kit
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Mouse Albumin ELISA Kit
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Mouse Albumin ELISA Kit

ELISA Standard Curve of Albumin using Mouse Albumin ELISA Kit (Product # EEL119). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Mouse Albumin ELISA Kit
Mouse Albumin ELISA Kit (EEL119)
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Product Specifications

Analytical sensitivity

37.5 ng/mL

Assay range

62.5-4,000 ng/mL

Sample type/volume

Serum
100 µL
Plasma
100 µL

Hands-on time

1 hr 20 min

Time-to-result

3 hr 30 min

Homogenous (no wash)

No

Interassay CV

<10%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer

Shipping conditions

Wet ice

Storage

Refer to product documentation for component specific storage temperature.

Protein name

Mouse Serum Albumin

Protein family

ALB/AFP/VDB

Species (tested)

Mouse

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Mouse Albumin ELISA quantitates Albumin in serum, plasma, and other biological fluids

The Mouse Albumin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation:

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : Alb, Alb-1, Alb1

Gene ID : (Mouse) 11657

Gene symbol : Alb

Protein Aliases : Albumin, albumin 1, serum albumin

UniProt ID (Mouse) P07724

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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