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Invitrogen

Mouse ANGPTL3 ELISA Kit

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Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Mouse ANGPTL3 ELISA Kit
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Mouse ANGPTL3 ELISA Kit
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Mouse ANGPTL3 ELISA Kit

ELISA Standard curve of ANGPTL3 using Mouse ANGPTL3 ELISA Kit (Product # EMANGPTL3). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Mouse ANGPTL3 ELISA Kit
Mouse ANGPTL3 ELISA Kit (EMANGPTL3)
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Product Specifications

Analytical sensitivity

40 pg/mL

Assay range

40.96-10,000 pg/mL

Sample type/volume

Plasma
1 µL
Serum
1 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

ANGPTL3

Protein family

Proteases Peptidases & Inhibitors

Species (tested)

Mouse

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Mouse ANGPTL3 ELISA quantitates Ms ANGPTL3 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms ANGPTL3.

Principle of the method

The Mouse ANGPLT3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

This gene encodes a member of a family of secreted proteins that function in angiogenesis. The encoded protein, which is expressed predominantly in the liver, is further processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metabolism, while the C-terminal chain may be involved in angiogenesis. Mutations in this gene cause familial hypobetalipoproteinemia type 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : Angptl3, hypl

Gene ID : (Mouse) 30924

Gene symbol : Angptl3

Protein Aliases : Angiopoietin-like protein 3, Angiopoietin-related protein 3, hypolipidemia

UniProt ID (Mouse) Q9R182

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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