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Invitrogen

Mouse 6-Ckine (CCL21A) ELISA Kit

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Technical guide & protocol Tech Support
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Technical guide & protocol Tech Support
Mouse 6-Ckine (CCL21A) ELISA Kit
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Mouse 6-Ckine (CCL21A) ELISA Kit
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Mouse 6-Ckine (CCL21A) ELISA Kit

ELISA Standard curve of 6-Ckine (CCL21A) using Mouse 6-Ckine (CCL21A) ELISA Kit (Product # EMCCL21A). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Mouse 6-Ckine (CCL21A) ELISA Kit
Mouse 6-Ckine (CCL21A) ELISA Kit (EMCCL21A)
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Product Specifications

Analytical sensitivity

20 pg/mL

Assay range

31.25-1,000 pg/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

CCL21

Protein family

Chemokines & Receptors

Species (tested)

Mouse

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Mouse 6-Ckine (CCL21A) ELISA quantitates Ms 6-Ckine in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms 6-Ckine.

Principle of the method

The Mouse 6-Ckine solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : 6CKBAC1, 6CKBAC2, 6Ckine, AI159701, ALP, AW987545, Ccl21a, Ccl21b, Ccl21c, CKb9, exodus-2, plt, Scya21, SCYA21a, Scya21b, Scya21c, SLC, TCA4

Gene ID : (Mouse) 18829, (Mouse) 100042493, (Mouse) 65956

Gene symbol : Ccl21a, Ccl21b, Ccl21c

Protein Aliases : 6 Ckine, 6Ckine, beta chemokine exodus-2, Beta-chemokine exodus-2, C-C motif chemokine 21a, C-C motif chemokine 21b, C-C motif chemokine 21c, CC chemokine 6Ckine-ser, Ccl21-leu, CCL21-Ser, exodus-2, secondary lymphoid organ chemokine, small inducible cytokine A21, small inducible cytokine A21a, small inducible cytokine A21c, Small-inducible cytokine A21a, Small-inducible cytokine A21b, Small-inducible cytokine A21c, TCA4, Thymus-derived chemotactic agent 4

UniProt ID (Mouse) P84444, (Mouse) P86792

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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