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Invitrogen

Human eNOS/NOS3 ELISA Kit

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Technical guide & protocol Tech Support
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Technical guide & protocol Tech Support
Human eNOS/NOS3 ELISA Kit
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Human eNOS/NOS3 ELISA Kit
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Human eNOS/NOS3 ELISA Kit

ELISA Standard curve of eNOS/NOS3 using Human eNOS/NOS3 ELISA Kit (Product # EH169RB). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human eNOS/NOS3 ELISA Kit
Human eNOS/NOS3 ELISA Kit (EH169RB)
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Product Specifications

Analytical sensitivity

0.4 ng/mL

Assay range

0.4-100 ng/mL

Sample type/volume

Supernatant
50 µL
Plasma
50 µL
Serum
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

eNOS

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human eNOS/NOS3 quantitates human eNOS/NOS3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human eNOS/NOS3.

Target information

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : ECNOS, eNOS, NOS3

Gene ID : (Human) 4846

Gene symbol : NOS3

Protein Aliases : cNOS, Constitutive NOS, e-NOS, OTTHUMP00000213183, EC-NOS, Endothelial NOS, Nitric oxide synthase 3, nitric oxide synthase 3 (endothelial cell), nitric oxide synthase 3 transcript variant eNOS-delta20, nitric oxide synthase 3 transcript variant eNOS-delta20-21, nitric oxide synthase 3 transcript variant eNOS-delta21, Nitric oxide synthase, endothelial, NOS type III, NOSIII

UniProt ID (Human) P29474

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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