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Invitrogen

Human VEGFC ELISA Kit

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Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Human VEGFC ELISA Kit
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Human VEGFC ELISA Kit
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Human VEGFC ELISA Kit

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Human VEGFC ELISA Kit
Human VEGFC ELISA Kit (BMS297-2)
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Product Specifications

Analytical sensitivity

0.057 ng/mL

Assay range

0.23-15.0 ng/mL

Sample type/volume

Plasma
20 µL
Serum
20 µL
Supernatant
20 µL

Hands-on time

1 hr 20 min

Time-to-result

2 hr 30 min

Homogenous (no wash)

No

Interassay CV

12%

Intraassay CV

6.7%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Lysis Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

VEGFC

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human Vascular Endothelial Growth Factor-C (Hu VEGF-C) ELISA quantitates Hu VEGF-C in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VEGF-C.

Principle of the method

The Human VEGF-C solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : Flt4-L, LMPH1D, VEGFC, VRP

Gene ID : (Human) 7424

Gene symbol : VEGFC

Protein Aliases : Flt4 ligand, FLT4 ligand DHM, Flt4-L, Vascular endothelial growth factor C, Vascular endothelial growth factor-related protein, VEGF-C, VRP, VEGF3

UniProt ID (Human) P49767

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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