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Invitrogen

Human VEGF-D (FIGF) ELISA Kit

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Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Human VEGF-D (FIGF) ELISA Kit
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Human VEGF-D (FIGF) ELISA Kit
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Human VEGF-D (FIGF) ELISA Kit

ELISA Standard curve of VEGF-D (FIGF) using Human VEGF-D (FIGF) ELISA Kit (Product # EHFIGF). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human VEGF-D (FIGF) ELISA Kit
Human VEGF-D (FIGF) ELISA Kit (EHFIGF)
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Product Specifications

Analytical sensitivity

0.2 ng/mL

Assay range

0.313-20 ng/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

VEGFD

Protein family

Chemokines & Receptors

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human Vascular Endothelial Growth Factor D (Hu VEGFD/FIGF) ELISA quantitates Hu VEGFD in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VEGFD.

Principle of the method

The Human VEGFD solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : FIGF, VEGF-D, VEGFD

Gene ID : (Human) 2277

Gene symbol : FIGF

Protein Aliases : c-fos induced growth factor (vascular endothelial growth factor D), c-Fos-induced growth factor, FIGF, Vascular endothelial growth factor D, VEGF-D, VEGF4

UniProt ID (Human) O43915

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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