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Human VAP-1 ELISA Kit

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Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Human VAP-1 ELISA Kit
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Human VAP-1 ELISA Kit
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Human VAP-1 ELISA Kit

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Human VAP-1 ELISA Kit
Human VAP-1 ELISA Kit (BMS259)
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Product Specifications

Analytical sensitivity

19 pg/mL

Assay range

31.3-2,000 pg/mL

Sample type/volume

Amniotic Fluid
10 µL
Plasma
10 µL
Serum
10 µL
Supernatant
10 µL

Hands-on time

1 hr 20 min

Time-to-result

3 hr 10 min

Homogenous (no wash)

No

Interassay CV

4.5%

Intraassay CV

3%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

AOC3

Target sub-specificity

Soluble

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human Vascular Adhesion Protein (Hu VAP-1) ELISA quantitates Hu VAP-1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VAP-1.

Principle of the method

The Human VAP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The product is a major protein on the adipocyte plasma membrane. It has adhesive properties and also has functional monoamine oxidase activity. A pseudogene for this gene has been discribed and is located approximately 9-kb downstream.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : AOC3, HPAO, SSAO, VAP-1, VAP1

Gene ID : (Human) 8639

Gene symbol : AOC3

Protein Aliases : Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3, amine oxidase, copper containing 3 (vascular adhesion protein 1), Copper amine oxidase, HPAO, placenta copper monamine oxidase, Semicarbazide-sensitive amine oxidase, SSAO, VAP-1, Vascular adhesion protein 1

UniProt ID (Human) Q16853

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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