Invitrogen
Human Thrombospondin 1 ELISA Kit
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Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
Hands-on time
Time-to-result
Homogenous (no wash)
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Intraassay CV
Instrument
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Contents
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
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Protein name
Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Human Thrombospondin-1 (Hu TSP-1) ELISA quantitates Hu TSP-1 in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu TSP-1.
Principle of the method
The Human TSP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : THBS, THBS-1, THBS1, TSP, TSP-1, TSP1
Gene ID : (Human) 7057
Gene symbol : THBS1
Protein Aliases : Glycoprotein G, Thrombospondin-1, thrombospondin-1p180, thrombospondin-p50, ThrombospondinTHBS1
UniProt ID (Human) P07996
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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