Invitrogen
Human TRAIL R3/ TNFRSF10C ELISA Kit
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Product Specifications
Analytical sensitivity
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Time-to-result
Homogenous (no wash)
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Contents
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
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Species (tested)
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Detector antibody conjugate
Label or dye
About This Kit
The Human TRAIL R3/TNFRSF10C (TRAIL R3) ELISA quantitates Hu TRAIL R3 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TRAIL R3.
Principle of the method
The Human TRAIL R3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
TRAIL-R3 (CD263) is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain and a transmembrane domain, but no cytoplasmic death domain. This receptor is not capable of inducing apoptosis, and is thought to function as an antagonistic receptor that protects cells from TRAIL-induced apoptosis. This gene was found to be a p53-regulated DNA damage-inducible gene. The expression of this gene was detected in many normal tissues but not in most cancer cell lines, which may explain the specific sensitivity of cancer cells to the apoptosis-inducing activity of TRAIL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : CD263, DCR1, DCR1-TNFR, LIT, TNFRSF10C, TRAIL-R3, TRAILR3, TRID, UNQ321/PRO366
Gene ID : (Human) 8794
Gene symbol : TNFRSF10C
Protein Aliases : Antagonist decoy receptor for TRAIL/Apo-2L, CD263, cytotoxic TRAIL receptor-3, DcR1, Decoy receptor 1, Decoy TRAIL receptor without death domain, Lymphocyte inhibitor of TRAIL, MGC149501, MGC149502, TNF-related apoptosis-inducing ligand receptor 3, TRAIL receptor 3, TRAIL receptor without an intracellular domain, Tumor necrosis factor receptor superfamily member 10C, tumor necrosis factor receptor superfamily, member 10c, decoy without an intracellular domain
UniProt ID (Human) O14755
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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