Invitrogen
Human TNFRSF10B (DR5) ELISA Kit
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Product Specifications
Analytical sensitivity
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Time-to-result
Homogenous (no wash)
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Intraassay CV
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Contents
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
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Species (tested)
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Detector antibody conjugate
Label or dye
About This Kit
The Human Tumor Necrosis Factor Receptor SuperFamily 10 B/Death Receptor 5 (TNFRSF10B (DR5)) ELISA quantitates Hu DR5 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu DR5.
Principle of the method
The Human DR5 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
DR5 (Apo2, TRAIL-R2, TRICK2, KILLER) is a recently identified death domain containing receptor for TRAIL, which mediates TRAIL induced apoptosis. DR5 is a member of the TNF-receptor superfamily, and contains an intracellular death domain. DR5 can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by DR5. Two transcript variants encoding different isoforms of DR5 and one non-coding transcript have been found. Overexpression of DR5 induces apoptosis and activates NF-kappa-B. DR5 is expressed in a number of cell types, and to particularly high levels in lymphocytes and spleen. Diseases associated with DR5 dysfunction include squamous cell carcinoma, and diffuse infiltrative lymphocytosis syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : CD262, DR5, KILLER, KILLER/DR5, TNFRSF10B, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, UNQ160/PRO186, ZTNFR9
Gene ID : (Human) 8795
Gene symbol : TNFRSF10B
Protein Aliases : apoptosis inducing protein TRICK2A/2B, apoptosis inducing receptor TRAIL-R2, CD262, cytotoxic TRAIL receptor-2, death domain containing receptor for TRAIL/Apo-2L, Death receptor 5, Fas-like protein, p53-regulated DNA damage-inducible cell death receptor(killer), sCD262, soluble CD262, dr-5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, Tumor necrosis factor receptor superfamily member 10B, tumor necrosis factor receptor superfamily, member 10b, tumor necrosis factor receptor-like protein ZTNFR9
UniProt ID (Human) O14763
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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