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Invitrogen

Human TARC/CCL17 ELISA Kit

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Technical guide & protocol Tech Support
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Technical guide & protocol Tech Support
Human TARC/CCL17 ELISA Kit
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Human TARC/CCL17 ELISA Kit
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Human TARC/CCL17 ELISA Kit

ELISA Standard curve of TARC/CCL17 using Human TARC/CCL17 ELISA Kit (Product # EHCCL17). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human TARC/CCL17 ELISA Kit
Human TARC/CCL17 ELISA Kit (EHCCL17)
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Product Specifications

Analytical sensitivity

5 pg/mL

Assay range

10.2-2,500 pg/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

TARC

Protein family

Chemokines & Receptors

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human TARC/CCL17 ELISA quantitates Hu TARC in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TARC.

Principle of the method

The Human TARC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

TARC ligand (CCL17) belongs to the CC chemokine family. It is a small cytokine and the gene is located on q arm of chromosome 16, in humans, along with other chemokines. TARC specifically binds to T-cells and interacts with chemokine receptors CCR4 and CCR8. It plays an important role in T cell development in thymus as well as in trafficking and activation of mature T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : A-152E5.3, ABCD-2, CCL17, SCYA17, TARC

Gene ID : (Human) 6361

Gene symbol : CCL17

Protein Aliases : C-C motif chemokine 17, CC chemokine TARC, Small-inducible cytokine A17, Thymus and activation-regulated chemokine, C-C motif chemokine, chemokine (C-C motif) ligand 17, small inducible cytokine subfamily A (Cys-Cys), member 17, T cell-directed CC chemokine

UniProt ID (Human) Q92583

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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