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Invitrogen

Human Sirtuin 3 ELISA Kit

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Technical guide & protocol Tech Support
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Technical guide & protocol Tech Support
Human Sirtuin 3 ELISA Kit
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Human Sirtuin 3 ELISA Kit
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Human Sirtuin 3 ELISA Kit

ELISA Standard curve of Sirtuin 3 using Human Sirtuin 3 ELISA Kit (Product # EH428RB). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human Sirtuin 3 ELISA Kit
Human Sirtuin 3 ELISA Kit (EH428RB)
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Product Specifications

Analytical sensitivity

2.1 ng/mL

Assay range

2.1-500 ng/mL

Sample type/volume

Serum
50 µL
Supernatant
50 µL
Plasma
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

SIRT3

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human Sirtuin 3 quantitates human Sirtuin 3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Sirtuin 3.

Target information

SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : SIR2L3, SIRT3

Gene ID : (Human) 23410

Gene symbol : SIRT3

Protein Aliases : HGNC:14931, hSIRT3, mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase, NAD-dependent deacetylase sirtuin-3, mitochondrial, NAD-dependent protein deacetylase sirtuin-3, NAD-dependent protein delactylase sirtuin-3, Regulatory protein SIR2 homolog 3, silent mating type information regulation 2, S.cerevisiae, homolog 3, sir2-like 3, SIR2-like protein 3, sirtuin type 3

UniProt ID (Human) Q9NTG7

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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