Invitrogen

Human SCF ELISA Development Kit (ABTS), PeproTech®

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Technical guide & protocol

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Human SCF ELISA Development Kit (ABTS)

Human SCF ABTS ELISA standard curve (Product # 900-K34K).

Human SCF ELISA Development Kit (ABTS) (900-K34K)

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Product Specifications

Analytical sensitivity

Lot-specific

Assay range

Lot-specific

Hands-on time

1 hr 45 min

Time-to-result

24 hr 30 min

Homogenous (no wash)

Instrument

Absorbance Microplate Reader

Product size

10 x 96 Tests

This Human SCF ELISA Kit includes Capture Antibody, Detection Antibody, Protein Standard, and HRP-conjugate. The HRP-conjugate is packaged separately.

Storage

Refer to product documentation for component specific storage temperature.

Protein name

SCF

Protein family

Growth Factors & Receptors

Species (tested)

Human

Assay kit format

ELISA Development Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

900-K34K consists of: 900-K34 (capture antibody, detection antibody, and protein standard), and AVIDIN-60UL (avidin HRP-conjugate).

Human SCF ABTS ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant human SCF in a sandwich ELISA format. Human SCF ABTS ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells.

The ABTS ELISA Buffer Kit (Product # 900-K00), sold separately, is recommended to be used in conjunction with this ABTS ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.

Target information

Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : DCUA, DFNA69, FPH2, FPHH, Kitl, KITLG, KL-1, MGF, SCF, SF, SHEP7

Gene ID : (Human) 4254

Gene symbol : KITLG

Protein Aliases : c-Kit ligand, DKFZp686F2250, H-SCF, kit-ligand, M-SCF, familial progressive hyperpigmentation 2, Kit ligand, Mast cell growth factor, MGF, SCF, steel factor, Stem cell factor

UniProt ID (Human) P21583

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