Invitrogen
Human S100B ELISA Kit
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Product Specifications
Analytical sensitivity
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Homogenous (no wash)
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Intraassay CV
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Protein name
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Detector antibody conjugate
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About This Kit
The Human S100 Calcium Binding Protein B (S100B) ELISA quantitates S100B in serum, plasma, and other biological fluids
The Human S100 Calcium Binding Protein B (S100B) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : NEF, S100, S100-B, S100B, S100beta
Gene ID : (Human) 6285
Gene symbol : S100B
Protein Aliases : Protein S100-B, S-100 calcium-binding protein, beta chain, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium binding protein beta (neural), S100 protein beta chain, S100 calcium-binding protein B, S100 calcium-binding protein, beta (neural)
UniProt ID (Human) P04271
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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