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Invitrogen

Human Procalcitonin ELISA Kit

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Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Human Procalcitonin ELISA Kit
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Human Procalcitonin ELISA Kit
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Human Procalcitonin ELISA Kit

ELISA Standard curve of Procalcitonin using Human Procalcitonin ELISA Kit (Product # EHPCT). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human Procalcitonin ELISA Kit
Human Procalcitonin ELISA Kit (EHPCT)
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Product Specifications

Analytical sensitivity

30 pg/mL

Assay range

27.43-20,000 pg/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

Procalcitonin

Protein subtype

Adaptor Proteins

Protein family

GPCR (G-Protein Coupled Receptors)

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human Procalcitonin ELISA quantitates Hu Procalcitonin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Procalcitonin.

Principle of the method

The Human Procalcitonin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Gene aliases : CALC1, CALCA, CGRP, CGRP-I, CGRP1, CT, KC, PCT

Gene ID : (Human) 796

Gene symbol : CALCA

Protein Aliases : Alpha-type CGRP, Calcitonin, calcitonin 1, Calcitonin carboxyl-terminal peptide, CCP, PDN-21, Calcitonin gene-related peptide 1, Calcitonin gene-related peptide I, calcitonin-related polypeptide alpha, calcitonin/calcitonin-related polypeptide, alpha, CGRP-I, CGRP1, katacalcin

UniProt ID (Human) P06881

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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