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Invitrogen

Human NrCAM ELISA Kit

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Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Human NrCAM ELISA Kit
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Human NrCAM ELISA Kit
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Human NrCAM ELISA Kit

ELISA Standard curve of NrCAM using Human NrCAM ELISA Kit (Product # EHNRCAM). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human NrCAM ELISA Kit
Human NrCAM ELISA Kit (EHNRCAM)
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Product Specifications

Analytical sensitivity

0.3 ng/mL

Assay range

0.328-80 ng/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

NRCAM

Protein family

Other Proteins

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human NrCAM ELISA quantitates Hu NrCAM in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NrCAM.

Principle of the method

The Human NrCAM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : KIAA0343, NRCAM

Gene ID : (Human) 4897

Gene symbol : NRCAM

Protein Aliases : hBravo, MGC138845, MGC138846, Nr CAM, Neuronal cell adhesion molecule, Neuronal surface protein Bravo, Ng-CAM-related, NgCAM-related cell adhesion molecule, Nr-CAM

UniProt ID (Human) Q92823

Recommended Products

IL-6 Human Matched Antibody Pair

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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