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Invitrogen

Human Nesfatin-1 ELISA Kit

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Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Human Nesfatin-1 ELISA Kit
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Human Nesfatin-1 ELISA Kit
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Human Nesfatin-1 ELISA Kit

ELISA Standard curve of Nesfatin-1 using Human Nesfatin-1 ELISA Kit (Product # EH333RB). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human Nesfatin-1 ELISA Kit
Human Nesfatin-1 ELISA Kit (EH333RB)
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Product Specifications

Analytical sensitivity

0.25 ng/mL

Assay range

0.25-60 ng/mL

Sample type/volume

Supernatant
50 µL
Serum
50 µL
Plasma
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

NUCB2

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human Nesfatin-1 quantitates human Nesfatin-1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Nesfatin-1.

Target information

NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : HEL-S-109, NEFA, NUCB2

Gene ID : (Human) 4925

Gene symbol : NUCB2

Protein Aliases : DNA-binding protein NEFA, Epididymis secretory protein Li 109, Gastric cancer antigen Zg4, nesfatin 1, nesfatin-1, Nucleobindin-2, Nucleobindin-2/Nesfatin-1, nucleobinding 2, prepronefastin, Prepronesfatin

UniProt ID (Human) P80303

Recommended Products

IL-6 Human Matched Antibody Pair

Because you are interested in IL-6 Human ELISA Kit.

SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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