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Invitrogen

Human MPIF-1/CCL23 ELISA Kit

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Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Human MPIF-1/CCL23 ELISA Kit
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Human MPIF-1/CCL23 ELISA Kit
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Human MPIF-1/CCL23 ELISA Kit

ELISA Standard curve of MPIF-1/CCL23 using Human MPIF-1/CCL23 ELISA Kit (Product # EHCCL23). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human MPIF-1/CCL23 ELISA Kit
Human MPIF-1/CCL23 ELISA Kit (EHCCL23)
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Product Specifications

Analytical sensitivity

7 pg/mL

Assay range

8.23-6,000 pg/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

CCL23

Protein family

GPCR (G-Protein Coupled Receptors)

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human MPIF-1/CCL23 ELISA quantitates Hu MPIF-1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MPIF-1.

Principle of the method

The Human MPIF-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils and no activity on activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. In addition, the product of this gene is a potent agonist of the chemokine (C-C motif) receptor 1. Alternative splicing results in multiple transcript variants that encode different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : CCL23, CK-BETA-8, Ckb-8, Ckb-8-1, CKb8, hmrp-2a, MIP-3, MIP3, MPIF-1, MPIF1, SCYA23

Gene ID : (Human) 6368

Gene symbol : CCL23

Protein Aliases : C-C motif chemokine 23, C6 beta-chemokine, chemokine (C-C motif) ligand 23, Ck beta 8-1, MIP-3CK-BETA-8, MPIF-1Ckb-8-1, CK-beta-8, CKB-8, Macrophage inflammatory protein 3, MIP-3, MPIF-1, Myeloid progenitor inhibitory factor 1, small inducible cytokine subfamily A (Cys-Cys), member 23, Small-inducible cytokine A23

UniProt ID (Human) P55773

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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