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Invitrogen

Human MIG/CXCL9 ELISA Kit

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Technical guide & protocol Tech Support
Human MIG/CXCL9 ELISA Kit
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Human MIG/CXCL9 ELISA Kit
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Human MIG/CXCL9 ELISA Kit

ELISA Standard curve of MIG/CXCL9 using Human MIG/CXCL9 ELISA Kit (Product # EHCXCL9). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human MIG/CXCL9 ELISA Kit
Human MIG/CXCL9 ELISA Kit (EHCXCL9)
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Product Specifications

Analytical sensitivity

20 pg/mL

Assay range

8.23-6,000 pg/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

CXCL9 (MIG)

Protein family

GPCR (G-Protein Coupled Receptors)

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human MIG/CXCL9 (Hu MIG) ELISA quantitates Hu MIG in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MIG.

Principle of the method

The Human MIG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

MIG, also known as CXCL9, is a T-cell chemoattractant inducible by gamma interferon that is a member of the CXC chemokine family of cytokines.This gene is mapped to 4q21. It is noted that, although the best-described activities of the chemokines are as chemotactic factors, chemokines also have an effect on T-cell activation, angiogenesis, and HIV infection. While most CXC chemokines are chemotactic for neutrophils, MIG and INP10 are unusual and similar in being CXC chemokines that are chemotactic for lymphocytes and inactive in neutrophils.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Gene aliases : CMK, crg-10, CXCL9, Humig, MIG, SCYB9

Gene ID : (Human) 4283

Gene symbol : CXCL9

Protein Aliases : C Cmotif chemokine, C X C motif chemokine, CC motif chemokine, CCmotif chemokine, CXC, CXC motif chemokine, CXCL, C-X-C motif chemokine 9, chemokine (C-X-C motif) ligand 9, Gamma-interferon-induced monokine, HuMIG, monokine induced by gamma interferon, Monokine induced by interferon-gamma, Small-inducible cytokine B9

UniProt ID (Human) Q07325

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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