Invitrogen
Human LIMP-II (SCARB2) ELISA Kit
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Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
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Time-to-result
Homogenous (no wash)
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Intraassay CV
Instrument
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Contents
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
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Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Human LIMP-II (SCARB2) ELISA quantitates Hu LIMP-II in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu LIMP-II.
Principle of the method
The Human LIMPM-II solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. SR-BI and SR-BII (previously known as SR-BI.2) are the alternatively spliced products of a single gene. SR-BII differs from SR-BI in that the encoded c-terminal cytoplasmic domain is almost completely different. SR-BII binds HDLs and mediates selective uptake of HDL cholesteryl ester, but with an approximately 4-fold lower efficiency than SR-BI. Nuclease protection assays show SR-BII to be abundant in mouse tissues expressing SR-BI, with SR-BII expression found in liver, adrenal glands, and testes. Although the role of SR-BII is not completely clear, research suggests that it may be a functional HDL receptor. In addition, SR-BII mRNA results from the alternative splicing of SR-BI precursor transcripts with the SR-BII isoform mediating selective transfer of lipid between HDL and cells. The relative expression and functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : AMRF, CD36L2, EPM4, HLGP85, LGP85, LIMP-2, LIMP2, LIMPII, SCARB2, SR-BII
Gene ID : (Human) 950
Gene symbol : SCARB2
Protein Aliases : 85 kDa lysosomal membrane sialoglycoprotein, 85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2, CD36, CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2 (lysosomal integral membrane protein II), CD36 antigen-like 2, LGP85, LIMP II, Lysosome membrane protein 2, Lysosome membrane protein II, Scavenger receptor class B member 2, scavenger receptor class B, SR-B2, CD 36, CD36 molecule, sCD 36, sCD36, soluble CD 36, soluble CD36
UniProt ID (Human) Q14108
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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