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Invitrogen

Human Kallikrein 14 ELISA Kit

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Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Human Kallikrein 14 ELISA Kit
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Human Kallikrein 14 ELISA Kit
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Human Kallikrein 14 ELISA Kit

ELISA Standard curve of Kallikrein 14 using Human Kallikrein 14 ELISA Kit (Product # EHKLK14). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human Kallikrein 14 ELISA Kit
Human Kallikrein 14 ELISA Kit (EHKLK14)
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Product Specifications

Analytical sensitivity

1.5 pg/mL

Assay range

2.05-500 pg/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

Kallikrein 14

Protein family

GPCR (G-Protein Coupled Receptors)

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human Kallikrein 14 (Hu KLK14) ELISA quantitates Hu KLK14 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu KLK14.

Principle of the method

The Human KLK14 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

Kallikreins are a subgroup of serine proteases and are implicated in carcinogenesis. A novel KLK gene, KLK12, is expressed in a variety of tissues including salivary gland, stomach, uterus, lung, thymus, prostate, colon, brain, thyroid, and trachea. It has applications as a novel cancer biomarker.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : KLK-L6, KLK14, KLKL6

Gene ID : (Human) 43847

Gene symbol : KLK14

Protein Aliases : hK14, Kallikrein-like protein 6, KLK-L6, Kallikrein L6, KLK-14, Kallikrein-14, kallikrein-related peptidase 14

UniProt ID (Human) Q9P0G3

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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