Invitrogen
Human IL-8 Instant ELISA™ Kit
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Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
Hands-on time
Time-to-result
Homogenous (no wash)
Interassay CV
Intraassay CV
Instrument
Product size
Contents
Biotinylated Detection Antibody
SAV-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Shipping conditions
Storage
Protein name
Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Human Interleukin 8 (Hu IL-8) ELISA quantitates Hu IL-8 in human serum, plasma, cell culture supernatants, amniotic fluid or other body fluids. The assay will exclusively recognize both natural and recombinant Hu IL-8.
Principle of the method
The Human IL-8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : CXCL8, GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1
Gene ID : (Human) 3576
Gene symbol : CXCL8
Protein Aliases : alveolar macrophage chemotactic factor I, ANX7, RP11-537A6.8, SNX, SYNEXIN, ILN, Interleukin, Interleukin8, beta endothelial cell-derived neutrophil activating peptide, beta-thromboglobulin-like protein, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, GCP-1, Granulocyte chemotactic protein 1, IL-8, interleukin 8, Interleukin-8, lung giant cell carcinoma-derived chemotactic protein, lymphocyte derived neutrophil activating peptide, MDNCF, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAP-1, neutrophil-activating peptide 1, Neutrophil-activating protein 1, Protein 3-10C, small inducible cytokine subfamily B, member 8, T-cell chemotactic factor, tumor necrosis factor-induced gene 1
UniProt ID (Human) P10145
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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