Invitrogen
Human IL-7 R alpha/CD127 ELISA Kit
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Product Specifications
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Homogenous (no wash)
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Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
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Detector antibody conjugate
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About This Kit
Human IL-7 R alpha/CD127 quantitates human IL-7 R alpha/CD127 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human IL-7 R alpha/CD127.
CD127, also known as the Interleukin-7 receptor alpha chain (IL-7Ralpha), is a type I glycoprotein with a molecular weight of 75-80 kDa. It forms a multi-functional receptor complex with CD132, the common gamma chain, to create the IL-7 receptor (IL-7R), which is crucial for lymphopoiesis. The active IL-7 receptor is an alpha/gamma chain heterodimer, where the gamma chain also associates with the interleukin-2 receptor and primarily activates signal transduction, while the alpha chain determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 is expressed by immature B cells through the early pre-B stage, thymocytes during various stages of development, and most mature T cells, with transient down-regulation upon activation. It promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. Binding of IL-7 to CD127 results in signal transduction through several tyrosine kinase pathways, including the Jak/STAT pathway, and is indispensable for lymphocyte development and the control of homeostatic proliferation of T cells in the periphery. Additionally, IL-7R signaling is involved in the regulation of T cell receptor (TCR) locus rearrangement in gamma delta T cells. Interestingly, CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (T regs). While CD4 and CD25 co-expression is commonly used to identify T regs, this method may include cells without suppressive activity. CD4+CD25+ cells that have down-regulated CD127 expression are significantly enriched for the regulatory T cell population, as defined by the expression of the T reg-specific transcription factor Foxp3 and their suppressive activity in vitro. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : CD127, CDW127, IL-7R-alpha, IL7R, IL7RA, ILRA
Gene ID : (Human) 3575
Gene symbol : IL7R
Protein Aliases : CD127, CD127 antigen, CDw127, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R, MGC107557, sCD127, soluble IL 7 receptor, soluble IL 7R, IL7R a, IL7R alpha, IL7R subunit alpha, IL7R α, IL7Rα, Interleukin7 receptor subunit alpha, IL 7R subunit alpha, IL 7R α, IL 7Ra, IL 7Rα, IL7 receptor, IL7 receptor subunit alpha, IL 7 receptor, IL 7R a, interleukin 7 receptor alpha chain, interleukin 7 receptor isoform H5-6, Interleukin-7 receptor subunit alpha
UniProt ID (Human) P16871
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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