Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
Hands-on time
Time-to-result
Homogenous (no wash)
Interassay CV
Intraassay CV
Instrument
Product size
Contents
Standard
Standard Dilution Buffer
Incubation Buffer
HRP conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Controls
Shipping conditions
Storage
Protein name
Protein family
Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Human Interleukin-12 (Hu IL-12 p70) ELISA quantitates Hu IL-12 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-12 heterodimer.
Principle of the method
The Human IL-12 p70 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : CLMF, CLMF2, IL-12A, IL-12B, IL12A, IL12B, IMD28, IMD29, NFSK, NKSF, NKSF1, NKSF2, P35
Gene ID : (Human) 3592, (Human) 3593
Gene symbol : IL12A, IL12B
Protein Aliases : 20C2, IL-12 p75, IL12A/B, IL 12p70, ILN, Interleukin, il 12, IL12AB, Interleukin-12 p70, MGC151228, MGC151232, CLMF p35, CLMF p40, cytotoxic lymphocyte maturation factor 1, p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p35, IL-12 subunit p40, IL-12, subunit p35, IL-12A, IL-12B, IL12, subunit p40, IL35 subunit, interleukin 12, p40, interleukin 12A (natural killer cell stimulatory factor 1, p35), interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), interleukin-12 alpha chain, interleukin-12 beta chain, Interleukin-12 subunit alpha, Interleukin-12 subunit beta, natural killer cell stimulatory factor, 40 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NK cell stimulatory factor chain 2, NKSF1, NKSF2
UniProt ID (Human) P29459, (Human) P29460
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support