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Invitrogen

Human I-309/CCL1 ELISA Kit

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Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Human I-309/CCL1 ELISA Kit
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Human I-309/CCL1 ELISA Kit
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Human I-309/CCL1 ELISA Kit

ELISA Standard curve of I-309/CCL1 using Human I-309/CCL1 ELISA Kit (Product # EHCCL1). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human I-309/CCL1 ELISA Kit
Human I-309/CCL1 ELISA Kit (EHCCL1)
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Product Specifications

Analytical sensitivity

4 pg/mL

Assay range

4.12-3,000 pg/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

CCL1

Protein family

Chemokines & Receptors

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human I-309/CCL1 ELISA quantitates Hu I-309 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu I-309.

Principle of the method

The Human I-309 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : CCL1, I-309, P500, SCYA1, SISe, TCA3

Gene ID : (Human) 6346

Gene symbol : CCL1

Protein Aliases : C-C motif chemokine 1, chemokine (C-C motif) ligand 1, inflammatory cytokine I-309, small inducible cytokine A1 (I-309, homologous to mouse Tca-3), Small-inducible cytokine A1, T lymphocyte-secreted protein I-309, CCL 1

UniProt ID (Human) P22362

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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