Invitrogen
Human FABP2 (intestinal) ELISA Kit
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Product Specifications
Analytical sensitivity
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Time-to-result
Homogenous (no wash)
Interassay CV
Intraassay CV
Instrument
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Contents
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
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Species (tested)
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Detector antibody conjugate
Label or dye
About This Kit
The Human FABP2 (intestinal) ELISA quantitates Hu FABP2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FABP2.
Principle of the method
The Human FABP2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
The FABP2 gene encodes the intestinal fatty acid-binding protein 2 (I-FABP), which plays a significant role in lipid metabolism by facilitating the transport and intracellular trafficking of long-chain fatty acids in enterocytes, the absorptive cells in the intestine. FABP2 is involved in the uptake, metabolism, and transfer of fatty acids across cellular membranes, contributing to digestion and absorption processes in the intestine. This protein is crucial for efficient lipid metabolism, influencing fatty acid oxidation, and energy balance. Variations in the FABP2 gene have been associated with metabolic traits, such as insulin sensitivity, lipid profiles, and obesity, suggesting its impact on metabolic health. FABP2's function in intestinal lipid absorption and metabolism makes it a subject of interest in studies related to dietary effects on health and the development of metabolic disorders. Understanding FABP2's role is essential for insights into nutritional influences on health and possible therapeutic targets for metabolic diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : FABP2, FABPI, I-FABP
Gene ID : (Human) 2169
Gene symbol : FABP2
Protein Aliases : fatty acid binding protein 2, intestinal, Fatty acid-binding protein 2, Intestinal-type fatty acid-binding protein, I-FABP, MGC133132, Fatty acid-binding protein
UniProt ID (Human) P12104
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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