Invitrogen
Human Estrogen Receptor beta ELISA Kit
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Product Specifications
Analytical sensitivity
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Sample type/volume
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Time-to-result
Homogenous (no wash)
Interassay CV
Intraassay CV
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Protein name
Species (tested)
Species (reported)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Human Estrogen Receptor Beta (ERBeta) ELISA quantitates ERBeta in serum, plasma and other biological fluids.
Principle of the method
The Human Estrogen Receptor Beta (ERb) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha. Some ER beta isoforms dominantly inhibit the activity of estrogen receptor alpha in reproductive tissues. ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : ER-BETA, Erb, ESR-BETA, ESR2, ESRB, ESTRB, NR3A2
Gene ID : (Human) 2100
Gene symbol : ESR2
Protein Aliases : ER, ER beta, ER-beta, estrogen receptor 2 (ER beta), Estrogen receptor beta, estrogen receptor beta 4, Nuclear receptor subfamily 3 group A member 2
UniProt ID (Human) Q92731
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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