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Invitrogen

Human ENA-78/CXCL5 ELISA Kit

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Technical guide & protocol Tech Support
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Technical guide & protocol Tech Support
Human ENA-78/CXCL5 ELISA Kit
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Human ENA-78/CXCL5 ELISA Kit
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Human ENA-78/CXCL5 ELISA Kit

ELISA Standard curve of ENA-78/CXCL5 using Human ENA-78/CXCL5 ELISA Kit (Product # EHCXCL5). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human ENA-78/CXCL5 ELISA Kit
Human ENA-78/CXCL5 ELISA Kit (EHCXCL5)
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Product Specifications

Analytical sensitivity

10 pg/mL

Assay range

24.69-18,000 pg/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

CXCL5

Protein family

Chemokines & Receptors

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human ENA-78/CXCL5 (ENA-78) ELISA quantitates Hu ENA-78 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ENA-78.

Principle of the method

The Human BTC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

The protein encoded by this gene is an inflammatory chemokine that belongs to the CXC chemokine family. This chemokine is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : CXCL5, ENA-78, ENA78, SCYB5

Gene ID : (Human) 6374

Gene symbol : CXCL5

Protein Aliases : C Cmotif chemokine, C X C motif chemokine, CC motif chemokine, CCmotif chemokine, CXC, CXC motif chemokine, CXCL, C-X-C motif chemokine 5, chemokine (C-X-C motif) ligand 5, ENA-78(1-78), Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, neutrophil-activating protein 78, small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78), Small-inducible cytokine B5

UniProt ID (Human) P42830

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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