Invitrogen
Human EGF ELISA Development Kit (ABTS), PeproTech®
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Product Specifications
Analytical sensitivity
Assay range
Hands-on time
Time-to-result
Homogenous (no wash)
Instrument
Product size
Contents
Storage
Protein name
Protein family
Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
900-K05K consists of: 900-K05 (capture antibody, detection antibody, and protein standard), and AVIDIN-60UL (avidin HRP-conjugate).
Human EGF ABTS ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant human EGF in a sandwich ELISA format. Human EGF ABTS ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells.
The ABTS ELISA Buffer Kit (Product # 900-K00), sold separately, is recommended to be used in conjunction with this ABTS ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : EGF, HOMG4, URG
Gene ID : (Human) 1950
Gene symbol : EGF
Protein Aliases : beta-urogastrone, EGF, H-EGF, Pro-epidermal growth factor
UniProt ID (Human) P01133
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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