Invitrogen
Human EG-VEGF/PROK1 ELISA Kit
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Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
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Time-to-result
Homogenous (no wash)
Interassay CV
Intraassay CV
Instrument
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Contents
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
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Protein name
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Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Human EG-VEGF/PROK1 ELISA quantitates Hu EG-VEGF in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu EG-VEGF.
Principle of the method
The Human EG-VEGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Endocrine gland-derived vascular endothelial growth factor induces proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. Its expression is induced by hypoxia and is restricted to the steroidogenic glands. Its expression is often complementary to the expression of VEGF, suggesting that these molecules function in a coordinated manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : EGVEGF, PK1, PRK1, PROK1, UNQ600/PRO1186
Gene ID : (Human) 84432
Gene symbol : PROK1
Protein Aliases : black mamba toxin-related protein, EG-VEGF, Endocrine-gland-derived vascular endothelial growth factor, Mambakine, Prokineticin-1
UniProt ID (Human) P58294
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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