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Human D-Dimer Rapid ELISA Kit

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Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Human D-Dimer Rapid ELISA Kit
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Human D-Dimer Rapid ELISA Kit
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Human D-Dimer Rapid ELISA Kit

ELISA Standard Curve of D-Dimer using Human D-Dimer Rapid ELISA Kit (Product # EELR023). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human D-Dimer Rapid ELISA Kit
Human D-Dimer Rapid ELISA Kit (EELR023)

Product Specifications

Analytical sensitivity

53.1 pg/mL

Assay range

125-4,000 pg/mL

Sample type/volume

Plasma
50 µL

Hands-on time

20 min

Time-to-result

3 hr 30 min

Homogenous (no wash)

No

Interassay CV

<10%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer

Shipping conditions

Wet ice

Storage

Refer to product documentation for component specific storage temperature.

Protein name

D-dimer

Species (tested)

Human

Species (reported)

Human

Assay kit format

Rapid ELISA Kit

Detector antibody conjugate

HRP

Label or dye

HRP

About This Kit

The Human D Dimer (D2D) ELISA quantitates D2D in plasma.

Principle of the method

The Human D2D solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells, where Human D2D in the samples (or standards) binds to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) HRP linked antibody specific for Human D2D. Excess conjugate and unbound sample or standard are washed away from the plate. The substrate solution is added to each well, and the enzyme substrate reaction is terminated by the addition of a stop solution, turning the color yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The intensity of this signal is directly proportional to the concentration of Human D2D present in the original specimen, which is determined by comparing the OD of the samples to the standard curve.

Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step.

Rigorous validation:

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Target information

Fibrinogen is the main protein of blood coagulation system. It consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. The process of blood coagulation results in the activation of fibrinogen into fibrin by thrombin and fibrin polymerization. Fibrin clot is then digested by plasmin, and fibrin degradation products of different molecular weights are released into the bloodstream.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : FGA, Fib2

Gene ID : (Human) 2243

Gene symbol : FGA

Protein Aliases : Fibrinogen alpha chain, fibrinogen, A alpha polypeptide, MGC119422, MGC119423, MGC119425

UniProt ID (Human) P02671

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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