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Invitrogen

Human Chitotriosidase ELISA Kit

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Technical guide & protocol Tech Support
Human Chitotriosidase ELISA Kit
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Human Chitotriosidase ELISA Kit
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Human Chitotriosidase ELISA Kit

ELISA Standard curve of Chitotriosidase using Human Chitotriosidase ELISA Kit (Product # EH105RB). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human Chitotriosidase ELISA Kit
Human Chitotriosidase ELISA Kit (EH105RB)
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Product Specifications

Analytical sensitivity

8 pg/mL

Assay range

8-2,000 pg/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

Chitotriosidase

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human Chitotriosidase quantitates human Chitotriosidase in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Chitotriosidase.

Target information

Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : CHI3, CHIT, CHIT1, CHITD

Gene ID : (Human) 1118

Gene symbol : CHIT1

Protein Aliases : chitinase 1 (chitotriosidase), Chitinase-1, Chitotriosidase, EC 3.2.1.14, FLJ00314, MGC125322, Chitotriosidase-1, plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase

UniProt ID (Human) Q13231

Recommended Products

IL-6 Human Matched Antibody Pair

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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