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Invitrogen

Human CTGF ELISA Kit

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Technical guide & protocol Tech Support
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Technical guide & protocol Tech Support
Human CTGF ELISA Kit
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Human CTGF ELISA Kit
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Human CTGF ELISA Kit

ELISA Standard Curve of CTGF using Human CTGF ELISA Kit (Product # EEL036). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human CTGF ELISA Kit
Human CTGF ELISA Kit (EEL036)
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Product Specifications

Analytical sensitivity

37.5 pg/mL

Assay range

62.5-4,000 pg/mL

Sample type/volume

Serum
100 µL
Plasma
100 µL

Hands-on time

1 hr 20 min

Time-to-result

3 hr 30 min

Homogenous (no wash)

No

Interassay CV

<10%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer

Shipping conditions

Wet ice

Storage

Refer to product documentation for component specific storage temperature.

Protein name

CTGF

Protein family

CCN

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human Connective Tissue Growth Factor (CTGF) ELISA quantitates CTGF in serum, plasma, and other biological fluids

The Human Connective Tissue Growth Factor solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation:

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

Connective Tissue Growth Factor (CTGF), also called CCN2, is a member of the CCN family. CTGF is a secreted protein produced by umbilical veins and vascular endothelial cells. CTGF possesses an Insulin-like Growth Factor (IGF)-binding domain, a thrombospondin type 1 domain, and a cysteine knot region. CTGF plays important roles in the proliferation and differentiation of chondrocytes, induces angiogenesis, and promotes cell adhesion in fibroblasts, endothelial, and epithelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : CCN2, CTGF, HCS24, IGFBP8, NOV2

Gene ID : (Human) 1490

Gene symbol : CTGF

Protein Aliases : 2-Nov, H-CTGF, MGC102839, CCN family member 2, Cellular communication network factor 2, Connective tissue growth factor, Hypertrophic chondrocyte-specific protein 24, IBP-8, IGF-binding protein 8, IGFBP-8, Insulin-like growth factor-binding protein 8

UniProt ID (Human) P29279

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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