Invitrogen
Human CKMB ELISA Kit
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Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
Hands-on time
Time-to-result
Homogenous (no wash)
Interassay CV
Intraassay CV
Instrument
Product size
Contents
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
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Protein name
Protein family
Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Human CKMB ELISA quantitates Hu CKMB in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CKMB.
Principle of the method
The Human CKMB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : B-CK, BCK, CKB, CKBB, CKM, CKMM, HEL-211, HEL-S-29, M-CK
Gene ID : (Human) 1152, (Human) 1158
Gene symbol : CKB, CKM
Protein Aliases : B-CK, Creatine kinase B chain, Creatine kinase M chain, M-CK, creatine kinase-M, CK MB, Brain creatine kinase, CPK-B, CPK-M, Creatine kinase B-type, creatine kinase brain, creatine kinase brain-type, Creatine kinase M-type, creatine kinase, brain, muscle, creatine kinase-B, Creatine phosphokinase B-type, Creatine phosphokinase M-type, epididymis luminal protein 211, epididymis secretory protein Li 29
UniProt ID (Human) P12277, (Human) P06732
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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