Invitrogen
Human CD63 ELISA Kit
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Product Specifications
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Homogenous (no wash)
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Detector antibody conjugate
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About This Kit
The Human TSPAN30/CD63 (Tetraspanin 30) ELISA quantitates TSPAN30/CD63 in serum, plasma, and other biological fluids
The Human TSPAN30/CD63 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
CD63, also known as LAMP-3, is a glycoprotein belonging to the tetraspanin family, primarily located in late endosomes, lysosomes, and secretory vesicles across various cell types. It is expressed intracellularly in resting platelets and basophils, but upon activation, CD63 translocates to the plasma membrane, making it a widely used marker for basophil activation. Unlike basophils, mast cells can expose CD63 without activation. CD63 is expressed on activated platelets, monocytes, macrophages, and granulocytes, as well as endothelial cells, fibroblasts, and smooth muscle cells. It interacts with integrins (such as VLA-3 and VLA-6) and TIMP-1, playing roles in mediating allergic responses, phagocytosis, and cell migration. Additionally, CD63 is involved in the regulation of H/K-ATPase trafficking and ROMK1 channels, and serves as a T-cell costimulation molecule. CD63 expression can be used to predict prognosis in early-stage carcinomas and is identical to the melanoma-associated antigen ME491 and the platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome, highlighting its significance in both immune responses and disease processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CD63, LAMP-3, ME491, MLA1, OMA81H, TSPAN30
Gene ID : (Human) 967
Gene symbol : CD63
Protein Aliases : CD 63, CD63, CD63 antigen, CD63 antigen (melanoma 1 antigen), Granulophysin, LAMP-3, Limp1, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, lysosome-associated membrane glycoprotein 3, Melanoma-associated antigen ME491, melanoma-associated antigen MLA1, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, Tspan-30
UniProt ID (Human) P08962
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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