Invitrogen
Human CD25/IL-2R (Soluble) Instant ELISA™ Kit
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Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
Hands-on time
Time-to-result
Homogenous (no wash)
Interassay CV
Intraassay CV
Instrument
Product size
Contents
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Shipping conditions
Storage
Protein name
Target sub-specificity
Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Human Soluble CD25/Interleukin 2 Receptor(Hu sCD25/IL-2R) ELISA quantitates Hu sCD25/IL-2R in human serum, plasma, urine or cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sCD25/IL-2R.
Principle of the method
The Human sCD25/IL-2R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : CD25, IDDM10, IL2R, IL2RA, IMD41, p55, TCGFR
Gene ID : (Human) 3559
Gene symbol : IL2RA
Protein Aliases : CD25, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2 RA antibody, Il2ra antibody, IL 2 RA, IL 2 receptor, IL 2R, IL 2R subunit alpha, soluble IL 2 receptor, il-2 receptor alpha, interleukin-2 receptor alpha, IL2 RA, IL2 receptor, IL2 receptor subunit alpha, IL2R subunit alpha, Interleukin2 receptor subunit alpha, sIL 2R, interleukin 2 receptor, alpha, Interleukin-2 receptor subunit alpha
UniProt ID (Human) P01589
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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