Invitrogen
Human Apolipoprotein C2 ELISA Kit
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Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
Hands-on time
Time-to-result
Homogenous (no wash)
Interassay CV
Intraassay CV
Instrument
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Contents
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
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Protein name
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Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Human Apolipoprotein C2 (Hu ApoC2) ELISA quantitates Hu ApoC2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ApoC2.
Principle of the method
The Human ApoC2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Apolipoproteins are protein components of plasma lipoproteins. The apolipoprotein C gene family encodes four homologous proteins designated apoC-I to -IV, which specifically modulate the metabolism of triglyceride-rich lipoproteins. The human apoC-I gene maps to chromosome 19q13.2 and is expressed primarily in the liver where it is activated when monocytes differentiate into macrophages. The human apoC-II gene maps to chromosome 19q13.2 and encodes a 79 amino acid single chain protein that is a necessary cofactor for the activation of lipoprotein lipase, the enzyme that hydrolyzes triglycerides in plasma and transfers the fatty acids to tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : APC2, APO-CII, APOC-II, APOC2
Gene ID : (Human) 344
Gene symbol : APOC2
Protein Aliases : Apo-CII, ApoC-II, Apolipoprotein C2, ProapoC-II, APC 2, Apo CII, APOC 2, ApoC II, APOC2 protein, ApoCII, APO C2, Apolipoprotein CII, Apolipoprotein C II, ApolipoproteinCII, MGC75082, Apolipoprotein C-II
UniProt ID (Human) P02655
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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