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Invitrogen

Human ADAM9 ELISA Kit

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Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Human ADAM9 ELISA Kit
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Human ADAM9 ELISA Kit
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Human ADAM9 ELISA Kit

ELISA Standard curve of ADAM9 using Human ADAM9 ELISA Kit (Product # EHADAM9). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human ADAM9 ELISA Kit
Human ADAM9 ELISA Kit (EHADAM9)
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Product Specifications

Analytical sensitivity

45 pg/mL

Assay range

49.15-12,000 pg/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

ADAM9

Protein family

Growth Factors & Receptors

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human ADAM9 ELISA quantitates Hu ADAM9 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ADAM9.

Principle of the method

The Human ADAM9 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

ADAM9 is a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This protein interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : ADAM9, CORD9, KIAA0021, MCMP, MDC9, MLTNG

Gene ID : (Human) 8754

Gene symbol : ADAM9

Protein Aliases : ADAM 9, ADAM metallopeptidase domain 9 (meltrin gamma), Cellular disintegrin-related protein, cone rod dystrophy 9, Disintegrin and metalloproteinase domain-containing protein 9, MDC 9, OTTHUMP00000216481, OTTHUMP00000216484, OTTHUMP00000216485, a disintegrin and metalloproteinase, ADAM, ADAMs, metalloendopeptidases, Meltrin-gamma, Metalloprotease/disintegrin/cysteine-rich protein 9, Myeloma cell metalloproteinase

UniProt ID (Human) Q13443

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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