Invitrogen
Human AChE ELISA Kit
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Product Specifications
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Homogenous (no wash)
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Intraassay CV
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Protein name
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Detector antibody conjugate
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About This Kit
The Human Acetylcholinesterase (AChE) ELISA quantitates Acetylcholinesterase in serum, plasma, and other biological fluids
The Human Acetylcholinesterase (AChE) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. The Protein is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : ACEE, ACHE, ARACHE, N-ACHE, YT
Gene ID : (Human) 43
Gene symbol : ACHE
Protein Aliases : Acetylcholinesterase, acetylcholinesterase (Yt blood group), AChE, apoptosis-related acetylcholinesterase, EC 3.1.1.7, OTTHUMP00000211349, OTTHUMP00000211356, Yt blood group
UniProt ID (Human) P22303
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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