Invitrogen
Cortisone Competitive ELISA Kit
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Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
Hands-on time
Time-to-result
Homogenous (no wash)
Interassay CV
Intraassay CV
Instrument
Product size
Contents
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Storage
Protein name
Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Cortisone ELISA quantitates Cortisone in serum, saliva, urine, plasm, or dried fecal extracts.
Principle of the method
The Cortisone Competitive ELISA research-use-only kit is designed to quantitatively measure cortisone in extracted dried fecal samples, urine, saliva, plasma, and serum samples. This kit measures total cortisone in serum and plasma and in extracted fecal samples. A cortisone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A cortisone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to cortisone to each well. After a two-hour incubation the plate is washed and TMB substrate is added. The substrate reacts with the bound cortisone-peroxidase conjugate to produce a colored product. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Cortisone (Kendall’s Compound E) is a glucocorticoid which is the product of cortisol (Compound F) deactivation by 11-beta-HSD2 (hydroxysteroid dehydrogenase), prohibiting receptor activation. The balance between cortisone and cortisol glucocorticoids helps to regulate the anti-inflammatory response. Cortisone is identical across all species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases : C21H28O5
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