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Invitrogen

Asymmetric Di-Methyl Arginine Competitive ELISA Kit

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Technical guide & protocol Tech Support
Asymmetric Di-Methyl Arginine Competitive ELISA Kit
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Asymmetric Di-Methyl Arginine Competitive ELISA Kit
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Asymmetric Di-Methyl Arginine Competitive ELISA Kit

ELISA Standard Curve of Asymmetric Di-Methyl Arginine using Asymmetric Di-Methyl Arginine Competitive ELISA Kit (Product # EEL009). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Asymmetric Di-Methyl Arginine Competitive ELISA Kit
Asymmetric Di-Methyl Arginine Competitive ELISA Kit (EEL009)
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Product Specifications

Analytical sensitivity

9.38 ng/mL

Assay range

15.63-1,000 ng/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL

Hands-on time

1 hr 20 min

Time-to-result

2 hr 30 min

Homogenous (no wash)

No

Interassay CV

<10%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer

Shipping conditions

Wet ice

Storage

Refer to product documentation for component specific storage temperature.

Protein name

Asymmetric Di-Methyl Arginine

Species (tested)

All

Assay kit format

Competitive ELISA

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The multispecies Asymmetrical Dimethylarginine (ADMA) ELISA quantitates ADMA in serum, plasma, and other biological fluids

Principle of the method:

The Competitive multispecies ELISA research-use-only kit is designed to quantitatively measure Asymmetrical Dimethylarginine (ADMA) independent of species. An ADMA standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to ADMA is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of ADMA in the samples is then determined by comparing the OD of the samples to the standard curve.

Rigorous validation:

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

Asymmetrical dimethylarginine (ADMA) is an L-arginine analogue twice methylated in an asymetric pattern. ADMA can be found naturally in the body and can contribute to endothelial and cardiovascular disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases : ADMA, Arginine

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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