Invitrogen
17-Hydroxyprogesterone Competitive ELISA Kit
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
Hands-on time
Time-to-result
Homogenous (no wash)
Interassay CV
Intraassay CV
Instrument
Product size
Contents
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Storage
Protein name
Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The 17-Hydroxyprogesterone Competitive ELISA quantitates 17-Hydroxyprogesterone in serum, plasma, urine, dried fecal extracts or cell culture medium.
Principle of the method
The 17-Hydroxyprogesterone Competitive ELISA research-use-only kit uses a specifically generated antibody to measure 17-Hydroxyprogesterone (17HO-P) and its metabolites in reconstituted buffer samples, tissue culture media samples, urine and fecal samples, or in extracted serum and plasma. This kit is not recommended for serum or plasma samples without extraction. A 17HO-P standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into the provided plate coated with a capture antibody. A 17HO-P-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to 17HO-P to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound 17HO-P-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
17-Hydroxyprogesterone (4-pregen-17-ol-3, 20-dione, 17HO-P, OHPG) is a steroid hormone from the androgen group that is found in mammals, reptiles, birds, and other vertebrates. 17HO-P is identical across all species and so this kit should measure 17HO-P from all sources. In humans, it is primarily synthesized in the adrenal glands, the gonads, testes, and placenta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases : C21H30O3, OHPG, 17-OH Progesterone
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support