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  • Primary Antibodies ›
  • p47phox Antibodies

Invitrogen

p47phox Polyclonal Antibody

View all (23) p47phox antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite p47phox Polyclonal Antibody

  • Antibody Testing Data (1)
p47phox Antibody in Western Blot (WB)
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p47phox Antibody in Western Blot (WB)
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p47phox Antibody (PA5-95502) in WB

Western blot analysis of p47phox in Lane 1: human HeLa whole cell lysates, Lane 2: human blood lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse NIH3T3 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with p47phox polyclonal antibody (Product # PA5-95502) using a 0.5 µg/mL dilution, follo... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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p47phox Antibody in Western Blot (WB)
p47phox Polyclonal Antibody

Product Details

PA5-95502

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

ELISA (ELISA)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human NCF1 recombinant protein (Position: M1-D270).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2807304

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human blood, rat thymus tissue, rat lung tissue, mouse NIH3T3 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

p47phox (NCF1) is required, along with NCF2 and membrane bound cytochrome b558, for activation of the latent NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in the gene can result in granulomatous disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NADPH oxidase subunit (47 kD); NADPH oxidase subunit (47kDa); NCF-1; NCF-47K; neutrop; Neutrophil cytosol factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; P47 phox; p47-phox; phagocyte oxidase 47 kDa; SH3 and PX domain-containing protein 1A

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Gene Aliases: Ncf-1; NCF-47K; NCF1; NCF1A; NOXO2; p47-phox; p47; p47phox; SH3PXD1A

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UniProt ID: (Human) P14598, (Mouse) Q09014

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Entrez Gene ID: (Human) 653361, (Mouse) 17969, (Rat) 114553

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Function(s)
protein binding electron carrier activity superoxide-generating NADPH oxidase activity superoxide-generating NADPH oxidase activator activity SH3 domain binding phosphatidylinositol binding phosphatidylinositol-3,4-bisphosphate binding lipid binding
Process(es)
response to yeast leukocyte mediated cytotoxicity respiratory burst involved in defense response protein targeting to membrane leukotriene metabolic process NADP catabolic process apoptotic process inflammatory response cellular defense response cell proliferation response to bacterium superoxide anion generation defense response to bacterium respiratory burst negative regulation of smooth muscle contraction hydrogen peroxide biosynthetic process defense response to Gram-positive bacterium defense response to fungus oxidation-reduction process neutrophil mediated killing of gram-positive bacterium neutrophil mediated killing of fungus antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent superoxide metabolic process positive regulation of catalytic activity innate immune response cell redox homeostasis vascular endothelial growth factor receptor signaling pathway
It has to be done as per old AB suggested Products section.
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