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  • Primary Antibodies ›
  • ASIC3 Antibodies

Osenses

extracellular ASIC3 Polyclonal Antibody

1 Reference
View all (16) ASIC3 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite extracellular ASIC3 Polyclonal Antibody

  • Antibody Testing Data (11)
extracellular ASIC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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extracellular ASIC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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FIGURE: 1 / 11

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extracellular ASIC3 Antibody (OSA00068W-100UL) in IHC (P)

IHC-P on paraffin sections of rat spinal cord (dorsal horn). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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extracellular ASIC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
extracellular ASIC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
extracellular ASIC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
extracellular ASIC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
extracellular ASIC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
extracellular ASIC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
extracellular ASIC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
extracellular ASIC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
extracellular ASIC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
extracellular ASIC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
extracellular ASIC3 Antibody in Western Blot (WB)
extracellular ASIC3 Polyclonal Antibody

Product Details

OSA00068W-100UL

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000
-

Miscellaneous PubMed (MISC)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / Ig

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from aa region 50-100 of rat ASIC3 conjugated to blue carrier protein was used as the antigen
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

The peptide is homologous in mouse.

Specificity of this antibody: ASIC3.

Target Information

ACCN3 encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, two hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. ACCN3 encodes a member that is an acid sensor and may play an important role in the detection of lasting pH changes. In addition, a heteromeric association between this member and ACCN1 has been observed as proton-gated channels sensitive to gadolinium.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: acid sensing ion channel 3; Acid-sensing ion channel 3; Amiloride-sensitive cation channel 3; ASIC3; Dorsal root ASIC; DRASIC; hASIC3; proton gated cation channel DRASIC

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Gene Aliases: Accn3; Asic3; AW742291; DRASIC; SLNAC1; TNAC1

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UniProt ID: (Mouse) Q6X1Y6, (Rat) O35240

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Entrez Gene ID: (Mouse) 171209, (Rat) 286920

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Function(s)
cation channel activity sodium channel activity ligand-gated sodium channel activity PDZ domain binding enterobactin transporter activity protein binding
Process(es)
transport ion transport cation transport sodium ion transport response to heat response to mechanical stimulus response to acidic pH ion transmembrane transport sodium ion transmembrane transport enterobactin transport detection of chemical stimulus involved in sensory perception sensory perception of sour taste detection of temperature stimulus involved in sensory perception detection of temperature stimulus involved in sensory perception of pain detection of mechanical stimulus involved in sensory perception of pain detection of chemical stimulus involved in sensory perception of pain detection of mechanical stimulus involved in sensory perception cation transmembrane transport
It has to be done as per old AB suggested Products section.
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