Invitrogen

eIF4H Polyclonal Antibody

Name: eIF4H Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-81167

View all (11) eIF4H antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)

eIF4H Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 2

eIF4H Antibody (PA5-81167) in ICC/IF

Immunofluorescence staining of eIF4H in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with eIF4H Polyclonal Antibody (Product # PA5-81167, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

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Product Details

PA5-81167

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunocytochemistry (ICC/IF)

1:300-1:10,000

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to the center region of the Human EIF4H

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2788402

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human EIF4H.

Target Information

This gene encodes one of the translation initiation factors, which functions to stimulate the initiation of protein synthesis at the level of mRNA utilization. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene generates 2 transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: eIF-4H; Eukaryotic translation initiation factor 4H; Williams-Beuren syndrome chromosomal region 1 protein; Williams-Beuren syndrome chromosome region 1

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Gene Aliases: eIF-4H; EIF4H; KIAA0038; WBSCR1; WSCR1

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UniProt ID: (Human) Q15056

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Entrez Gene ID: (Human) 7458

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Function(s)

nucleotide binding RNA binding translation initiation factor activity helicase activity protein binding translation factor activity, RNA binding RNA strand annealing activity RNA strand-exchange activity ribosomal small subunit binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion

Process(es)

formation of translation preinitiation complex cytoplasmic translation translational initiation regulation of translational initiation viral process sexual reproduction developmental growth eukaryotic translation initiation factor 4F complex assembly cell-cell adhesion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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eIF4H Polyclonal Antibody

eIF4H Antibody (PA5-81167) in ICC/IF

Product Details

PA5-81167

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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