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  • eIF4B Antibodies

Invitrogen

eIF4B Monoclonal Antibody (1F5)

View all (28) eIF4B antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite eIF4B Monoclonal Antibody (1F5)

  • Antibody Testing Data (5)
eIF4B Antibody in Immunocytochemistry (ICC/IF)
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eIF4B Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 5

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eIF4B Antibody (MA5-17071) in ICC/IF

Immunofluorescence analysis of NIH3T3 cells using EIF4B monoclonal antibody (Product # MA5-17071) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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eIF4B Antibody in Immunocytochemistry (ICC/IF)
eIF4B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
eIF4B Antibody in Western Blot (WB)
eIF4B Antibody in Western Blot (WB)
eIF4B Antibody in Flow Cytometry (Flow)

Product Details

MA5-17071

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000
-

Immunocytochemistry (ICC/IF)

1:200-1:1,000
-

Flow Cytometry (Flow)

1:200-1:400
-

ELISA (ELISA)

1:10,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

1F5

Immunogen

Purified recombinant fragment of human EIF4B expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538542

Product Specific Information

MA5-17071 targets EIF4B in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Mouse samples.

The MA5-17071 immunogen is purified recombinant fragment of human EIF4B expressed in E. Coli.

MA5-17071 detects EIF4B which has a predicted molecular weight of approximately 80kDa.

Target Information

The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: eIF-4B; Eukaryotic translation initiation factor 4B; hCG_2014609

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Gene Aliases: 2310046H11Rik; AL024095; C85189; EIF-4B; Eif4a2; EIF4B; PRO1843

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UniProt ID: (Human) P23588, (Mouse) Q8BGD9

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Entrez Gene ID: (Human) 1975, (Mouse) 75705

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Function(s)
nucleotide binding RNA binding translation initiation factor activity helicase activity protein binding RNA strand annealing activity RNA strand-exchange activity ribosomal small subunit binding poly(A) RNA binding nucleic acid binding
Process(es)
nuclear-transcribed mRNA poly(A) tail shortening formation of translation preinitiation complex cytoplasmic translation translational initiation regulation of translational initiation eukaryotic translation initiation factor 4F complex assembly translation
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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