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  • Primary Antibodies ›
  • eIF4B Antibodies

Invitrogen

eIF4B Polyclonal Antibody

View all (28) eIF4B antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite eIF4B Polyclonal Antibody

  • Antibody Testing Data (3)
eIF4B Antibody in Immunocytochemistry (ICC/IF)
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eIF4B Antibody in Immunocytochemistry (ICC/IF)
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eIF4B Antibody (PA5-92449) in ICC/IF

Immunofluorescence analysis of eIF4B in A549 cells. Samples were incubated with eIF4B Polyclonal antibody (Product # PA5-92449). Blue: DAPI for nuclear staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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eIF4B Antibody in Immunocytochemistry (ICC/IF)
eIF4B Antibody in Western Blot (WB)
eIF4B Antibody in Immunoprecipitation (IP)
eIF4B Polyclonal Antibody

Product Details

PA5-92449

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:50-1:100
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_0014082)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2806507

Product Specific Information

Immunogen sequence: SQSTRAASIF GGAKPVDTAA REREVEERLQ KEQEKLQRQL DEPKLERRPR ERHPSWRSEE TQERERSRTG SESSQTGTST TSSRNARRRE SEKSLENETL NKEEDCHSPT SKPPKPDQPL KVMPAPPPKE NAWVKRSSNP PARSQSSDTE QQSPTSGGGK VAPAQPSEEG PGRKDENKVD GMNAPKGQTG NSSRGPGDGG NRDHWKESDR KDGKKDQDSR SAPEPKKPEE NPASKFSSAS KYAALSVDGE DENEGEDYAE; Positive Samples: 22Rv1, HeLa, 293T, M21

Target Information

The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: eIF-4B; Eukaryotic translation initiation factor 4B; hCG_2014609

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Gene Aliases: 2310046H11Rik; AL024095; C85189; EIF-4B; Eif4a2; EIF4B; PRO1843

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UniProt ID: (Human) P23588, (Mouse) Q8BGD9

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Entrez Gene ID: (Human) 1975, (Mouse) 75705

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Function(s)
nucleotide binding RNA binding translation initiation factor activity helicase activity protein binding RNA strand annealing activity RNA strand-exchange activity ribosomal small subunit binding poly(A) RNA binding nucleic acid binding
Process(es)
nuclear-transcribed mRNA poly(A) tail shortening formation of translation preinitiation complex cytoplasmic translation translational initiation regulation of translational initiation eukaryotic translation initiation factor 4F complex assembly translation
It has to be done as per old AB suggested Products section.
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