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  • eIF2 gamma Antibodies

Invitrogen

eIF2 gamma Polyclonal Antibody

4 Published Figures
4 References
View all (9) eIF2 gamma antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite eIF2 gamma Polyclonal Antibody

  • Antibody Testing Data (1)
  • Published Figures (4)
eIF2 gamma Antibody in Western Blot (WB)
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eIF2 gamma Antibody in Western Blot (WB)
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eIF2 gamma Antibody (PA5-31177) in WB

Western Blot using eIF2 gamma Polyclonal Antibody (Product # PA5-31177). Sample (30 µg of whole cell lysate). Lane A: HepG2. 10% SDS PAGE. eIF2 gamma Polyclonal Antibody (Product # PA5-31177) diluted at 1:1,000. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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eIF2 gamma Antibody in Western Blot (WB)
eIF2 gamma Antibody in Immunohistochemistry (IHC)
eIF2 gamma Antibody in Western Blot (WB)
eIF2 gamma Antibody in Western Blot (WB)
eIF2 gamma Antibody in Western Blot (WB)

Product Details

PA5-31177

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 3 publications 3 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Goat, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF2S3. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2548651

Product Specific Information

Recommended positive controls: HeLa, HepG2, HCT116.

Predicted reactivity: Rat (100%), Zebrafish (98%), Xenopus laevis (97%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex is composed of three subunits, designated eIF2 alpha, eIF2 beta and eIF2 gamma (eukaryotic translation initiation factor 2 alpha, beta and gamma, respectively), all of which work in concert to form a ternary complex with GTP and tRNA in the early stages of protein synthesis. eIF2S3 (eukaryotic translation initiation factor 2, subunit 3), also known as eIF2G, is a 472 amino acid protein that belongs to the gamma subfamily of GTP-binding elongation factor proteins. Existing as a heterotrimer composed of an alpha, beta and gamma chain, eIF2S3 functions to bind ribosomal subunits and catalyze the subsequent formation of preinitiation complexes necessary for protein synthesis.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: eIF-2-gamma X; eIF-2gX; eIF2-gamma X; Eukaryotic translation initiation factor 2 subunit 3; Eukaryotic translation initiation factor 2 subunit gamma X; eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa; eukaryotic translation initiation factor 2G

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Gene Aliases: eIF-2gA; EIF2; EIF2G; EIF2gamma; EIF2S3

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UniProt ID: (Human) P41091

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Entrez Gene ID: (Human) 1968

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Function(s)
translation initiation factor activity GTPase activity protein binding GTP binding translation factor activity, RNA binding cadherin binding involved in cell-cell adhesion
Process(es)
formation of translation preinitiation complex translational initiation transmembrane transport cell-cell adhesion
It has to be done as per old AB suggested Products section.
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