Invitrogen

beta Tubulin Loading Control Monoclonal Antibody (BT7R), DyLight™ 800 4X PEG

Name: beta Tubulin Loading Control Monoclonal Antibody (BT7R), DyLight\u2122 800 4X PEG
Brand: Invitrogen
SKU: MA5-16308-D800

1 Reference

View all (69) beta Tubulin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

beta Tubulin Loading Control Antibody in Western Blot (WB)

FIGURE: 1 / 1

beta Tubulin Loading Control Antibody (MA5-16308-D800) in WB

Western blot analysis of beta-Tubulin was performed by loading 75 µg of the indicated cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a low fluorescence PVDF membrane (Product # 22860) and blocked with SEA BLOCK Blocking Buffer (Product # 37527) for at least 1 hour. The membrane was probed with a DyLight 800-conjugated beta-Tubulin monoclonal antibody (Product # MA5-16308... View More

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Immunogen image

Immunogen Image beta Tubulin Loading Control Monoclonal Antibody (BT7R), DyLight™ 800 4X PEG

Product Details

MA5-16308-D800

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

BT7R

Immunogen

KLH conjugated peptide of the N-terminus of Beta-Tubulin.

View immunogen

Conjugate

DyLight™ 800 4X PEG DyLight™ 800 4X PEG DyLight™ 800 4X PEG

Excitation/Emission Max

789/794 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

MES with proprietary stabilizer

Contains

no preservative

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2537826

Product Specific Information

MA5-16308-D800 detects bet-Tubulin from various species. MA5-16308-D800 has been successfully used for fluorescent detection of beta-Tubulin in Western Blotting.

DyLight 800 4X PEG has an excitation/emission of 783/797 nm.

Target Information

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: beta Ib tubulin; beta tubulin 1; beta-4 tubulin; beta-tubulin T beta15 (aa 1-445); betaIII-tubulin; class II beta-tubulin isotype; class IIa beta-tubulin; class IIb beta-tubulin; class III beta-tubulin; class V beta-tubulin; dJ40E16.7; dystonia 4, torsion (autosomal dominant); HSA10p15 beta-tubulin 4Q; M40 antibody; Neuron-specific class III beta-tubulin; T beta-15; tubb2a {ECO:0000250|UniProtKB:Q7TMM9}; Tubulin 5 beta; tubulin beta; Tubulin beta 8 class VIII; Tubulin beta chain; Tubulin beta class IIa; Tubulin beta class V; tubulin beta MGC4083; Tubulin beta-1 chain; Tubulin beta-2A chain; Tubulin beta-2B chain; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4A chain; Tubulin beta-5 chain; Tubulin beta-6 chain; Tubulin beta-8 chain; Tubulin beta-III; tubulin, beta 1 class VI; tubulin, beta 2; tubulin, beta 2A class IIa; tubulin, beta 2B class IIb; tubulin, beta 3 class III; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 4A class IVa; tubulin, beta 6 class V; tubulin, beta class I; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta polypeptide paralog; tubulin, beta, 5

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Gene Aliases: 2310057H16Rik; 2410129E14Rik; 2810484G07Rik; 3200002H15Rik; AA408537; AI325297; AI596182; B130022C14Rik; bA506K6.1; bA631M21.2; BB220206; beta-4; beta-5; brdp; CDCBM; CDCBM1; CDCBM5; CDCBM6; CFEOM3; CFEOM3A; CSCSC1; DYT4; FEOM3; HsT1601; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; M40; OK/SW-cl.56; OOMD; OOMD2; PMGYSA; RGD1305887; RGD1309427; TUBB; TUBB-5; TUBB1; TUBB2; TUBB2A; TUBB2B; TUBB3; TUBB4; TUBB4A; TUBB5; TUBB6; TUBB8