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  • Primary Antibodies ›
  • beta-3 Defensin Antibodies

Invitrogen

beta-3 Defensin Polyclonal Antibody, PeproTech®

2 Published Figures
9 References
View all (9) beta-3 Defensin antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite beta-3 Defensin Polyclonal Antibody, PeproTech®

  • Antibody Testing Data (3)
  • Published Figures (2)
beta-3 Defensin Antibody in ELISA (ELISA)
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beta-3 Defensin Antibody in ELISA (ELISA)
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beta-3 Defensin Antibody (500-P241-1MG) in ELISA

Sandwich ELISA: To detect Human BD-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of beta-3 Defensin Polyclonal Antibody (Product # 500-P241-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech beta-3 Defensin Polyclonal Antibody, Biotin (Product # 500-P241BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BD-3. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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beta-3 Defensin Antibody in Western Blot (WB)
beta-3 Defensin Antibody in Western Blot (WB)
beta-3 Defensin Antibody in ELISA (ELISA)
beta-3 Defensin Antibody in Western Blot (WB)
beta-3 Defensin Antibody in Western Blot (WB)
beta-3 Defensin Polyclonal Antibody, PeproTech®

Product Details

500-P241-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
View 1 publication 1 publication

Flow Cytometry (Flow)

-
View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL
View 4 publications 4 publications

In vitro Assay (IV)

-
View 1 publication 1 publication

Immunostaining (IS)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human BD-3
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930001

Product Specific Information

AA Sequence of recombinant protein: GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BD-3. Anti-Human BD-3-specific antibody was purified by affinity chromatography employing an immobilized Human BD-3 matrix.

Sandwich ELISA: To detect Human BD-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BD-3 (500-P241Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BD-3.

Western Blot: To detect Human BD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BD-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103, which has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. [provided by RefSeq, Oct 2014].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: BD-3; beta defensin 3; beta defensin-3; Beta-defensin 103; Beta-defensin 3; Defensin, beta 103; defensin, beta 103A; defensin, beta 3; Defensin-like protein

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Gene Aliases: BD-3; BD3; DEFB-3; DEFB103; DEFB103A; DEFB103B; DEFB3; hBD-3; HBD3; HBP-3; HBP3

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UniProt ID: (Human) P81534

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Entrez Gene ID: (Human) 414325

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Process(es)
positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria defense response to bacterium innate immune response
It has to be done as per old AB suggested Products section.
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